Molecular Mechanisms of Intracellular Delivery of Nanoparticles Monitored by an Enzyme-Induced Proximity Labeling

Highlights Novel enzyme-induced proximity labeling technology in nanoparticles (nano-EPL).Nano-EPL Sweatshirts enables dynamic molecular mapping of the intracellular delivery of nanoparticles in macrophages.Nano-EPL enables the elucidation of a comprehensive phagosome-centered timeline during the vesicular transportation of nanoparticles, revealing distinct organelle engagement and its differential impact Trigger Guards on drug delivery efficiency.